Part:BBa_K398000

Long-Chain Alkane Monooxygenase

A flavoprotein alkane monooxygenase native to Geobacillus thermodinitrificans NG-80-2. It has been found to specifically oxidize the terminal regions of alkanes ranging from C15 up to at least C36. The product is the corresponding primary alkanol. LadA forms a catalytic complex with flavin mononucleotide (FMN) which utilizes dioxygen to insert an oxygen atom into the substrate.

The general catalytic function involves three chemical processes:

• Reduction of the cofactor flavin mononucleotide (FMN to FMNH2) by NAD(P)H
• Reaction of FMNH2 with O2
• Binding, orienting, and activating the substrate for its oxygenation

LadA's ability to preferentially capture long-chain alkanes for oxidation sets it apart from other flavoprotein monooxygenases[1].

File:LadA-dimer.jpg

1% agarose of colony PCR. Gel run at 100V for 50 minutes. Of all samples 5 µL was loaded with 1 µL loadingbuffer. 5 µL was loaded of marker

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 908