Reporter

Part:BBa_K341002

Designed by: Sheng WANG   Group: iGEM10_Tsinghua   (2010-10-20)
Revision as of 20:07, 5 November 2010 by Aobom (Talk | contribs)

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promoter+tet-SDS

tet-SDS gene together with Lac-promoter working as a reporter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1147
  • 1000
    COMPATIBLE WITH RFC[1000]


Safety

This part is for research use only, no special safety issues need to be mentioned.

References

Thomas E. Kuhlman and Edward C. Cox:Site-specific chromosomal integration of large synthetic constructs,Nucleic Acids Research, 2009, 1–10

Acknowledgement

The physical DNA is from Vecter "pTKs-CS" which is generously provided by Thomas E. Kuhlman and Edward C. Cox of the Department of Molecular Biology, Princeton University.

We are grateful to Guoqiang Chen, Zhao Wang,Yinghua Chen and Liping Xie of School of Life Sciences, Tsinghua University for assistance in the design, construction and characterization of this part.



[edit]
Categories
Parameters
biologyWork as a Landing Pad fragment
directionForward
functionLanding Pad Fragment in the In-vivo Recombnation System
originArtifical
outputTet resistance
resistanceTet