Composite

Part:BBa_K358019:Design

Designed by: Ken Kajita   Group: iGEM10_Kyoto   (2010-10-19)
Revision as of 11:49, 23 October 2010 by Tako (Talk | contribs) (→‎Design Notes)

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Lysis cassette regulated by lacP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

K358019.jpg

Because of the lysis cassette, lactose promoter must be repressed when transform it. So, we used low copy plasmid, pSB4K5, and then transformed it to KRX, not Top 10. It has lacI in the genome. See KRX genotype here, [http://www.promega.com/pnotes/94/14410_27/14410_27.pdf].

Source

biobrick[BBa_R0011], lambda phage DNA[http://catalog.takara-bio.co.jp/PDFFiles/3403_DS_j.pdf].

References