Coding

Part:BBa_K317003:Design

Designed by: Yoshiyuki MURAKAMI   Group: iGEM10_Tokyo-NoKoGen   (2010-10-19)
Revision as of 21:11, 27 October 2010 by Yoshiyuki (Talk | contribs) (→‎Design Notes)

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NpSRII-9a.a.linker_NpHtrII-StTar


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1789
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 331
    Illegal AgeI site found at 1591
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1027
    Illegal BsaI.rc site found at 1306
    Illegal SapI site found at 801
    Illegal SapI.rc site found at 1807


Design Notes

The coding sequence was amplified with primer sets which were designed based on 'Standard part fabrication' described in http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication

Phototaxis Device-2.png Primer sets.png

Source

Natronomonas pharaonis (Obtained from National Institute of Technology and Evaluation(NBRC). (NBRC 14720T)) Salmonella Typhimurium

References

Jung,et al.(2001)J.Bacteriol.183,6365–6371