Part:BBa_K346034

T7p(BBa_I719005)+RBS(B0034)+Lpp-OmpA+MBP(surface display of mercury binding peptide)

This part is the combination of a T7promoter(BBa_I719005),RBS(B0034)and Lpp-OmpA+MBP(surface display of mercury binding peptide).It functions as the surface display part of MBP in our project.

Description

It is highly expected that when fused with N-terminal fragment of Outer membrane protein A (OmpA), the mercury metal binding peptide(MBP)will be translocated and displayed on the surface of bacteria, which will contribute a lot to increase the mental binding capacity when combined will other collection parts.

Lpp-OmpA-mbp is designed as a fusion protein consisting of the signal sequence and first 9 amino acid of Lpp, residue 46~159 of OmpA and the metal binding peptide(MBP). The signal peptide of the N-termini of this fusion protein targets the protein on the membrane while the trans-membrane domain of Ompa serves as an anchor. MBP is on the externally exposed loops of OmpA, which can be anchored to the outer membrane.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 660
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]