Coding
virA

Part:BBa_K389001:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-08-10)
Revision as of 13:42, 4 October 2010 by Jaretz (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

VirA receptor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 586
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1571
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1707


Design Notes

An illegal PstI restriction site was removed by site-directed mutagenesis, the startcodon was changed to ATG and the stop codon to TAA. A second TAA stop codon was added.

Source

Agrobacterium tumefaciens C58, TI-plasmid

References