Part:BBa_K352004

G2C Mutated pCooF from Rhodospirillum rubrum

This part is the mutated version of pCooF promoter. The mutation involves conversion guanine to cytosine at second base. The binding strength of pCooF to CooA is expected to diminish.

Rhodospirillum rubrum is able to use CO as an carbon source with its CO-sensing protein called CooA. CooA activates the transcription of genes encoding the CO-oxidation (coo) regulon which produces the proteins needed for the utilization of CO. CooA binds to a position overlapping the -35 element of the P(cooF) promoter, similar to the arrangement of class II CRP (cAMP receptor protein)- and FNR (fumarate and nitrate reductase activator protein)-dependent promoters when expressed in Escherichia coli.

The two CO regulated transcriptional units, cooMKLXUH and cooFSCTJ, is responsible for the CO-dependent anaerobic growth of Rhodospirillum rubrum and enable the bacteria to oxidize CO . The coo regulon has three main products which are listed as O2-sensitive CO dehydrogenase (CooS), CooS-associated Fe-S protein (CooF), CO-tolerant hydrogenase (CooH). CooA is responsible for the expression of the genes of these proteins and activates by sensing CO in anaerobic environments.

Because of the lack of active CooA, CO-independent basal level transcription of PcooF is not detectable.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]