Coding

Part:BBa_K305005:Design

Designed by: Maarten van den Nieuwenhof, Ramon Sieber, Arend Jan Suk   Group: iGEM10_Groningen   (2010-09-27)
Revision as of 18:31, 27 October 2010 by Jorrithillebrand (Talk | contribs) (→‎Design Notes)

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Sortase srtA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RBS sequence used in this part can be found in several B. subtilis genes and is known to efficiently initiate translation. The spacer sequence between the RBS and start codon originates form the B. subtilis wapA gene. The CDS was optimized for B. subtilis by the GenScript OptimumGeneTM software (see images).

Frequency of optimal codons before optimization. The x-axis 100 value is set for the codon with the highest usage frequency for a given amino acid in B. subtilis.
Frequency of optimal codons after optimization. The x-axis 100 value is set for the codon with the highest usage frequency for a given amino acid in B. subtilis.


Source

GenBank: AF162687.1

References