Regulatory

Part:BBa_K385005:Design

Designed by: Stephen Lam, Justyna Kucia, Joseph Hoare, Krystal Annand   Group: iGEM10_Aberdeen_Scotland   (2010-09-27)
Revision as of 12:22, 27 September 2010 by I.stansfield (Talk | contribs) (→‎Design Notes)

B-box sequence encoding a regulatory mRNA stem loop


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence forms part of a 5' untranslated leader, and so must be free from all AUG sequences. The stem sequence itself was; GGGCCCTGAAGAAGGGCC

It was flanked on the 5' side by; 5' ATTATCTACTTAA 3' and on the 3' side by; 5' CTTAAGAACACAAAATTCGAGACAT G 3' (note this is 'sense' strand sequence)

Source

Phage lambda genome

References