Translational_Unit
sacB

Part:BBa_K322921:Design

Designed by: Chris French and Maria Kowal   Group: iGEM10_Edinburgh   (2010-09-24)
Revision as of 16:07, 25 October 2010 by WillR (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

MABEL was used to mutate an internal EcoRI site to GAGTTC.

Source

Source: Bacillus subtilis 168 genomic DNA. Sequence (from SacBPlan11June10.docx)


References