Translational_Unit
Part:BBa_K322451:Design
Designed by: William Rostain Group: iGEM10_Edinburgh (2010-09-01)
Photinus pyralis luciferase mutated for increased bioluminescence.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1281
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1417
Illegal NgoMIV site found at 1458
Illegal AgeI site found at 1150 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Wild type sequence with three mutations which increase relative bioluminescence.
Source
DNA was synthesised to have the same amino acids as the wild type except for the three mutations.