Signalling

Part:BBa_F2620

Designed by: Barry Canton [bcanton@mit.edu] and Anna Labno [labnoa@mit.edu]   Group: iGEM04_MIT   (2004-08-09)
Revision as of 02:13, 5 June 2006 by Registry (Talk | contribs)


3OC6HSL -> PoPS Receiver

This device senses the 3OC6HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the tet repressible R0040. Initial characterization work has been done on this device using I13273 as a test device.


Usage and Biology

<img src="Include File F2620Data"

The transfer function indicates a switch point of approximately 2nM which was constant in repeated experiments (data not shown).

The latency shows that fluorescent proteins appear within moments of the addition of AHL to the media. This suggests that PoPS output adjusts to a change in input very rapidly. This is expected given the current model of device operation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


[edit]
Categories
//classic/signalling/receiver
//function/cellsignalling
Parameters
controlR0040
device_typeReceiver
family
latencySeconds
proteinsLuxR, TetR
signalling_molecule3OC6HSL
switch_point2nM