Reporter

Part:BBa_J70626

Designed by: Joseph Lynch   Group: Knight Lab   (2010-08-11)
Revision as of 14:32, 11 August 2010 by Jolynch (Talk | contribs)

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RFC12 His tagged RFP

This is a N-terminal fusion of a his tag onto a RFP protein, with the requisite stop codons. This part will cause cells to grow red, but if you are looking for a His-tagged RFP without the stop codons (i.e. for fusions), this part will not work. Either use BBa_J70595 (GFP), BBa_J70609, or some combination of the two if you wish to have a GFP and His tag respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 587
    Illegal AgeI site found at 699
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None