Coding

Part:BBa_J72031:Design

Designed by: Jennifer Brophy   Group: Anderson Lab   (2010-07-15)
Revision as of 22:35, 15 July 2010 by Jennbrophy (Talk | contribs) (→‎Source)

{Bmll64} in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll64 {<HSV!}

Wobble mll64/mll65                (70, EcoRI/BamHI
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll64         {<HSV!}
----
mll64    Forward wobble for {<HSV!} (Bmll64)
CCATAGAATTCATGAGATCTCAGCCGGAGCTCGCACCAGAAGACC
mll65    Reverse wobble for {<HSV!} (Bmll64)
CGTTAGGATCCttaGCAGTCTTCCGGGTCTTCTGGTGCGAGC

References