Coding

Part:BBa_K367002:Design

Designed by: Fernando Govantes   Group: iGEM10_UPO-Sevilla   (2010-06-29)
Revision as of 13:37, 25 October 2010 by Fgovrom (Talk | contribs) (→‎Design Notes)

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prhI coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 114
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 91
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Start codon changed from GTG to ATG and stop codon changed to TAATAA. Codon usage changed to match E. soli using Mr. Gene's tools. Purchased from Mr. Gene

Source

Ralstonia solanacearum GMI1000 plasmid pGMI1000MP NCBI Reference Sequence: NC_003296.1

References