Coding

Part:BBa_K367001:Design

Designed by: Fernando Govantes   Group: iGEM10_UPO-Sevilla   (2010-06-29)
Revision as of 13:35, 25 October 2010 by Fgovrom (Talk | contribs) (→‎Design Notes)

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prhR coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 559
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 858
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Start codon changed from GTG to ATG and stop codon changed to TAATAA. Codon usage changed to match E. coli with Mr. Gene's tools. Purchased from Mr. Gene

Source

Ralstonia solanacearum GMI1000 plasmid pGMI1000M NCBI Reference Sequence: NC_003296.1

References