Part:BBa_K5051000:Design

PKS1

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1012
  Illegal BglII site found at 1504
  Illegal BglII site found at 4192
  Illegal BglII site found at 5371
  Illegal BglII site found at 5677
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 2939
  Illegal SapI.rc site found at 5579
  

Design Notes

It was codon optimized for Saccharomyces cerevisiae

Source

The PKS1 gene is from Aureobasidium pullulans EXF-150 genomic sequence

References

1. Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., Bridgland, A., Meyer, C., Kohl, S. a. A., Ballard, A. J., Cowie, A., Romera-Paredes, B., Nikolov, S., Jain, R., Adler, J., . . . Hassabis, D. (2021). Highly accurate protein structure prediction with AlphaFold. Nature, 596(7873), 583–589. https://doi.org/10.1038/s41586-021-03819-2

2. Mirdita, M., Schütze, K., Moriwaki, Y., Heo, L., Ovchinnikov, S., & Steinegger, M. (2022). ColabFold: making protein folding accessible to all. Nature Methods, 19(6), 679–682. https://doi.org/10.1038/s41592-022-01488-1

3. Xue, S., Liu, G., Chi, Z., Gao, Z., Hu, Z., & Chi, Z. (2020). Genetic evidences for the core biosynthesis pathway, regulation, transport and secretion of liamocins in yeast-like fungal cells. Biochemical Journal, 477(5), 887–903. https://doi.org/10.1042/bcj20190922