Intermediate

Part:BBa_K5051006:Design

Designed by: Yuk Ho Tsang   Group: iGEM24_PLKNPL-HK   (2024-10-02)
Revision as of 13:08, 2 October 2024 by Thegodofsheep (Talk | contribs)

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Kozak


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 26


Design Notes

Source

References

1. Dvir, S., Velten, L., Sharon, E., Zeevi, D., Carey, L. B., Weinberger, A., & Segal, E. (2013). Deciphering the rules by which 5′-UTR sequences affect protein expression in yeast. Proceedings of the National Academy of Sciences, 110(30). https://doi.org/10.1073/pnas.1222534110

2. Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015). A highly characterized Yeast toolkit for modular, multipart assembly. ACS Synthetic Biology, 4(9), 975–986. https://doi.org/10.1021/sb500366