Regulatory

Part:BBa_K264049:Design

Designed by: George McArthur IV   Group: iGEM09_Virginia_Commonwealth   (2009-11-10)
Revision as of 03:10, 11 November 2009 by GMcArthurIV (Talk | contribs) (→‎Design Notes)

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K264003.J23119 (UP/promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 31
    Illegal NheI site found at 54
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A spacer, CT, was placed at the end of the UP-element to serve as a 2 bp spacer between the UP-element and promoter. There is no scar between the UP element and promoter.

Source

This is a composite part.

References