Composite
Open loop

Part:BBa_K255004

Designed by: Navneet Rai   Group: iGEM09_IIT_Bombay_India   (2009-10-13)
Revision as of 16:47, 31 October 2009 by Nrai (Talk | contribs)

pTet.lacI-cfp(fusion).terminator.pTet.yfp.terminator

Network without any feedback and feedforward loop, present in pSB1A2 vector. BBa_K255004 represents a open loop system with constitutive expression of LacI and replication of plasmid copy. Both LacI and plasmid copy number are independent of IPTG concentration (inducer). The copy number is characterized by YFP while LacI is characterized by LacI-CFP fusion protein. YFP was measured using FACS and the distribution obtained was independent of IPTG. Beta-galactosidase was also measured at various lactose concentration to characterize the expression of gene in the host genome. Further the growth of the transformant was also characterized at different lactose concentrations.

A
Expression of BBa_K255004 in lacI deleted E.coli strain


Applications of BBa_K255004

This part was used as open loop system in our experiments.

Safety

Because this part is from commonly used elements in bacteria. No safety problem can be arised by this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1161
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apTet.lacI-cfp(fusion).terminator.pTet.yfp.terminator