Device

Part:BBa_K249017:Design

Designed by: Roxanne Shank   Group: iGEM09_Lethbridge   (2009-08-26)
Revision as of 15:05, 31 October 2009 by Roxanne (Talk | contribs) (→‎Design Notes)

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N-terminal YFP --> Tet inverter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Standard Prefix and Suffix, although there is BioFusion (aka Silver Lab) scar sequence fusing the YFP gene to the N-terminal Arginine Tag

Source

BioBrick Registry and synthesized Arganine Tag

References