Device

Part:BBa_K249014:Design

Designed by: Roxanne Shank   Group: iGEM09_Lethbridge   (2009-08-26)
Revision as of 14:57, 31 October 2009 by Roxanne (Talk | contribs) (→‎Design Notes)

C-terminal YFP --> Tet inverter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Standard Prefix and Suffix, although there is BioFusion (aka Silver Lab) scar sequence fusing the YFP gene to the C-terminal Arginine Tag

This part was designed as an inversion device for YFP fluorescence. Upon addition of Arabinose the fluorescence of the protein was expected to drop significantly. This was observed.

File:Results fig 11.png File:Results fig 21.png

Source

BioBrick Registry and synthesized Arganine Tag

References