Coding

Part:BBa_K5477018

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-26)
Revision as of 23:42, 1 October 2024 by Kateesc1700 (Talk | contribs)

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UDPD - UDP-glucose dehydrogenase

UDP-glucose dehydrogenase (UDPD) biosynthesizes UDP-glucuronic acid from UDP-Glucose. UDP-glucuronic acid is a needed substrate in the phase II detoxification reactions carried out by UDP-glucuronosyltransferase (UGT). Saccharomyces cerevisiae has a large pool of UDP-glucose but lacks the ability to synthesize UDP-glucoronic acid (1). This sequence is from Arabidopsis thaliana - At1g26570/T1K7_6.

Due to the use of UGTs in the detoxification modules, UDPD is co-expressed to provide the necessary substrate for the glucuronidation reactions where UGT1A1 and UGT2B15 were used (1) (2). The following composites and devices, where this part was used, are listed below: BBa_K5477035, BBa_K5477036, BBa_K5477040 and BBa_K5477046 and BBa_K5477047.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 542
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 542
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1122
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 542
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 542
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Oka T, Jigami Y. Reconstruction of de novo pathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose in Saccharomyces cerevisiae. FEBS J. 2006 Jun;273(12):2645–57.

2. Rowland A, Miners JO, Mackenzie PI. The UDP-glucuronosyltransferases: their role in drug metabolism and detoxification. Int J Biochem Cell Biol. 2013;45(6):1121-1132. doi:10.1016/j.biocel.2013.02.019

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