Coding

Part:BBa_K2619004

Designed by: shiyu liu   Group: iGEM18_XJTLU-CHINA   (2018-09-08)
Revision as of 20:36, 1 October 2024 by Nicoc123 (Talk | contribs)

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19kDa nano luciferase/nanoLuc

This Nano luciferase (nluc) is the mutated catalytic 19 kDa component of Oplophorus luciferase. nluc is a newly developed luciferase. In our project, it can be expressed as an indicator of exosome quantity by linking it with CD63 and lamp2b. We use it as reporter of membrane protein gene expression. It is also combined with C/D box and 3 stem loop to exam the transportation efficiency of packaging devices.


Usage and Biology

Team UVU-Utah-2, 2024

We obtained this part from the Chlamydomonas MoClo kit as described in Crozet et al. (2018). We utilized and characterized this part in composite part(s) K5078003. Please check out the documentation for that composite part for more details!


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 230
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None