Part:BBa_K5175039:Design

T7 promoter-tpaK-T7 terminator

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 919
  Illegal PstI site found at 1208
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1475
  Illegal PstI site found at 919
  Illegal PstI site found at 1208
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 919
  Illegal PstI site found at 1208
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 919
  Illegal PstI site found at 1208
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

It is a composite component consisting of the T7 promoter, T7 terminator, target gene tpaK. It is responsible for transporting TPA. TpaK is the TPA transporter protein.

Source

tpak is from Rhodococcus erythropolisRHA1.

References

[1] KINCANNON W M, ZAHN M, CLARE R, et al. Biochemical and structural characterization of an aromatic ring-hydroxylating dioxygenase for terephthalic acid catabolism [J]. Proc Natl Acad Sci U S A, 2022, 119(13): e2121426119. [2] VERMAAS J V, DIXON R A, CHEN F, et al. Passive membrane transport of lignin-related compounds [J]. Proc Natl Acad Sci U S A, 2019, 116(46): 23117-23. [3] HOSAKA M, KAMIMURA N, TORIBAMI S, et al. Novel tripartite aromatic acid transporter essential for terephthalate uptake in Comamonas sp. strain E6 [J]. Appl Environ Microbiol, 2013, 79(19): 6148-55.