Part:BBa_K5127002:Design

BreR

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 400
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 400
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 400
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 400
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E. coli.

Source

Genome of Vibrio cholerae.

References

Abramson, J., Adler, J., Dunger, J., Evans, R., Green, T., Pritzel, A., Ronneberger, O., Willmore, L., Ballard, A. J., Bambrick, J., Bodenstein, S. W., Evans, D. A., Hung, C. C., O'Neill, M., Reiman, D., Tunyasuvunakool, K., Wu, Z., Žemgulytė, A., Arvaniti, E., ... Jumper, J. M. (2024). Accurate structure prediction of biomolecular interactions with AlphaFold 3. Nature, 630(493-500). https://doi.org/10.1038/s41586-024-07487-w

Beabout, K., Ehrenworth Breedon, A. M., Blum, S. M., Miklos, A. E., Lux, M. W., Chávez, J. L., & Goodson, M. S. (2022). Detection of Bile Acids in Complex Matrices Using a Transcription Factor-Based Biosensor. ACS Biomaterials Science & Engineering, 9(9), 5151–5162. https://doi.org/10.1021/acsbiomaterials.2c01006