Part:BBa_K5127000:Design

VFA0359

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 250
  Illegal SapI.rc site found at 544
  

Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E. coli.

Source

Genome of Vibrio fischeri.

References

d’Oelsnitz, S., Stofel, S. K., Love, J. D., & Ellington, A. D. (2024). Snowprint: a predictive tool for genetic biosensor discovery. Communications Biology, 7(1). https://doi.org/10.1038/s42003-024-05849-8

LaFleur, T. L., Hossain, A., & Salis, H. M. (2022). Automated model-predictive design of synthetic promoters to control transcriptional profiles in bacteria. Nature Communications, 13(1). https://doi.org/10.1038/s41467-022-32829-5

Mutalik, V. K., Guimaraes, J. C., Cambray, G., Lam, C., Christoffersen, M. J., Mai, Q.-A., Tran, A. B., Paull, M., Keasling, J. D., Arkin, A. P., & Endy, D. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements. Nature Methods, 10(4), 354–360. https://doi.org/10.1038/nmeth.2404

Ruegg, T. L., Pereira, J. H., Chen, J. C., DeGiovanni, A., Novichkov, P., Mutalik, V. K., Tomaleri, G. P., Singer, S. W., Hillson, N. J., Simmons, B. A., Adams, P. D., & Thelen, M. P. (2018). Jungle Express is a versatile repressor system for tight transcriptional control. Nature Communications, 9, 3617. https://doi.org/10.1038/s41467-018-05857-3

Taketani, M., Zhang, J., Zhang, S., Triassi, A. J., Huang, Y. J., Griffith, L. G., & Voigt, C. A. (2020). Genetic circuit design automation for the gut resident species Bacteroides thetaiotaomicron. Nature Biotechnology, 38(9), 962–969. https://doi.org/10.1038/s41587-020-0468-5