Part:BBa_K5136231

J23106-SD7-LexRO-B0015-pColE408-ccdB-B0014-pHybrid 2)-114 version-B0034-ccdA

Biology

pColE48 is a promoter to which LexRO (BBa_K5136042) binds and represses the expression of genes driven by the promoter. The gene ccdB encodes a toxic protein (CcdB) that, as a DNA gyrase poison, locks DNA gyrase together with broken double-stranded DNA, ultimately leading to cell death. Ｔhe gene ccdA is found within the ccd operon, encoding the antidote protein (CcdA) that protects cells from the toxic effects of CcdB. Lonprotease easily degrades CcdA protein. The cell loses the ccdA gene due to the loss of the F plasmid, causing the cell to succumb to the toxicity of CcdB.

Usage

To verify the performance of blue light induced kill switch, we designed a composite part: BBa_K5136231.

Figure 1 Gene circuit of BBa_K5136231

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 1607
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 456
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1376