Composite
Delay test

Part:BBa_K257013:Experience

Designed by: Soufiane Boumahdi   Group: iGEM09_Paris   (2009-10-17)
Revision as of 03:58, 22 October 2009 by Benoît dH (Talk | contribs) (Applications of BBa_K257013)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K257013


PbadLacIYFP trans-1.jpg
x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar without glucose neither arabinose


PbadLacIYFP fluo.jpg
x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction on LB agar without glucose neither arabinose


PbadLacIYFP glucose trans1.jpg
x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar with glucose


PbadLacIYFP glucose fluo1.jpg
x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction plated on LB agar with glucose


Conclusion :
In presence of glucose pBad is repressed and then no fluorescence is normally observed. Accordingly to the construction less bacteria with our construction are fluorescent in presence of glucose than in the control.