Plasmid

Part:BBa_K243034

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-20)
Revision as of 19:38, 23 October 2009 by Tobias (Talk | contribs)

pJS418


Expression vector with a strong Shine-Dalgarno sequence designed for E. coli expression strains.


Freiburg09 pJS418.JPG

Usage and Biology

The pJS419 vector is an expression vector with a strong RBS, it is useful for the expression of non-toxic genes and products which do not easily aggregate at a high expression level. The plasmid confers chloramphenicol resistance to the host cell allowing a positive selection.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2267
    Illegal XbaI site found at 1383
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2268
    Illegal PstI site found at 2282
    Illegal NotI site found at 2275
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1495
    Illegal BamHI site found at 2366
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2268
    Illegal XbaI site found at 1383
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal suffix found in sequence at 2258
    Illegal XbaI site found at 1383
    Illegal NgoMIV site found at 1391
    Illegal NgoMIV site found at 2499
    Illegal AgeI site found at 3833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2115


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Categories
Parameters
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