Plasmid

Part:BBa_K5302037

Designed by: Dekun Zhou   Group: iGEM24_USTC   (2024-10-01)
Revision as of 09:05, 1 October 2024 by Hagibis (Talk | contribs)


pBBR-OmpA-l2VGB

This work is derived from pBBR-OmpA-VEGFR1D2 and pUC19-VGB, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and VEGFR1D2(approximately 12kda), and it adds a fragment as a mask. We succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2379
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2379
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
    Illegal NotI site found at 5311
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2379
    Illegal BglII site found at 6057
    Illegal BamHI site found at 1302
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2379
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2379
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 857
    Illegal NgoMIV site found at 3037
    Illegal AgeI site found at 1955
    Illegal AgeI site found at 2877
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5633
    Illegal SapI.rc site found at 423
    Illegal SapI.rc site found at 633


[edit]
Categories
//plasmid
Parameters
biologyEscherichia coli Nissle 1917