Part:BBa_K5108003

Creatinase from Pseudomonas putida

Creatinase from Pseudomonas putida

Contents
1. Usage and Biology
2. Characterization and Measurements
3. References

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 225
  Illegal BsaI site found at 1012
  

Usage and Biology

Creatinase (CreA) is an enzyme that catalyzes the degradation of creatine into sarcosine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (creA) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct in BBa_K5108009.

Characterization and Measurements

SDS-PAGE, growth analysis and consumption analysis of creatinine by NMR spectroscopy were performed. The results can be found in BBa_K5108009 and in iGEM ToulouseINSA-UPS 2024.

References

UniProt. (s. d.-b). https://www.uniprot.org/uniprotkb/P38488/entry

Cloning, Expression and Purification of Pseudomonas putida ATCC12633 Creatinase. (2017, 1 décembre). PubMed. https://pubmed.ncbi.nlm.nih.gov/29090065/