Part:BBa_K5115051

ribozyme connected hox and hyp

Introduction

This composite part combines all the subunit of the hydrogenase in our ribozyme-assisted polycistronic co-expression system：pRAP. To learn more about the hydrogenase, please check BBa_K5115020(hox and hyp operon). To get more information about pRAP, please check part wiki of 2022 Fudan iGEM .

Characterization

Agarose gel electrophoresis

Figure 1. Agarose gel electrophoresis of PCR products amplified from one E. coli (DH5α) colony. M: DNA Marker. Lanes 1-8: Corresponding bands for hoxF, hoxU, hoxI, hoxH, hoxW, hoxY, hypA, and hypB, demonstrating successful assembly and integrity of the ribozyme-connected hox and hyp operon as designed. Primers for these PCR are listed on https://2024.igem.org/fudan/parts.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 654
  Illegal BglII site found at 1472
  Illegal BglII site found at 1765
  Illegal BglII site found at 2412
  Illegal BglII site found at 2490
  Illegal BamHI site found at 2883
  Illegal XhoI site found at 99
  Illegal XhoI site found at 2420
  Illegal XhoI site found at 2612
  Illegal XhoI site found at 3090
  Illegal XhoI site found at 5329
  Illegal XhoI site found at 7158
  Illegal XhoI site found at 8320
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 533
  Illegal NgoMIV site found at 1071
  Illegal NgoMIV site found at 1281
  Illegal NgoMIV site found at 1593
  Illegal NgoMIV site found at 3183
  Illegal NgoMIV site found at 3863
  Illegal NgoMIV site found at 6470
  Illegal AgeI site found at 2373
  Illegal AgeI site found at 7645
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2194
  Illegal BsaI site found at 2356
  Illegal BsaI site found at 4983
  Illegal BsaI.rc site found at 702
  Illegal BsaI.rc site found at 1206
  Illegal SapI.rc site found at 2305