Plasmid

Part:BBa_K5322031

Designed by: Xinquan Zhou   Group: iGEM24_NJTech-China   (2024-10-01)
Revision as of 13:10, 1 October 2024 by Baiyun (Talk | contribs)


Programmed Cell Death 1 Ligand 1 Functional Domain [Mus musculus] Expression System


Usage and Biology

The complete mouse PD-L1 protein has a large molecular weight and complex structure, making it challenging to express in the prokaryotic organism Escherichia coli. Therefore, we opted for a truncated version of the PD-L1 functional domain, designing an expression system for the PD-L1 functional domain: pET29a-J23119-RBS-PD-L1 (functional domain)-T7. The plasmid diagram is shown below.

pET29a-J23119-RBS-PD-L1 Functional domain -T7

Figure 1-1 Plasmid pET29a-J23119-RBS-PD-L1 Functional domain -T7

Construction of the plasmid

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 377
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 519
    Illegal PstI site found at 377
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 277
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 377
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 377
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None