Plasmid

Part:BBa_K5322030:Design

Designed by: Xinquan Zhou   Group: iGEM24_NJTech-China   (2024-10-01)
Revision as of 12:49, 1 October 2024 by Baiyun (Talk | contribs) (→‎Design Notes)


Programmed Cell Death 1 Ligand 1 [Mus musculus] Expression System


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 356
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 314
    Illegal NheI site found at 939
    Illegal PstI site found at 356
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 205
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 356
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 356
    Illegal NgoMIV site found at 745
    Illegal AgeI site found at 577
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon optimization was performed based on the codon usage table of Escherichia coli.

Source

123

References