Coding

Part:BBa_K5302001

Designed by: Jiacan Ma   Group: iGEM24_USTC   (2024-09-30)
Revision as of 08:17, 1 October 2024 by Xiaotian (Talk | contribs)


8IIU

8IIU is an anti-VEGF nanobody, and it has 2 chains, chainA sequence: mdvqlvesgg glvqpggslr lscaasgrtf ssysmgwfrq apgkerefvv aiskggykyd avslegrfti srdnakntvy lqinslrped tavyycassr aygssrlrla dtyeywgqgt lvtvss. chainB sequence:mdvqlvesgg glvqpggslr lscaasgrtf ssysmgwfrq apgkerefvv aiskggyky avslegrfti srdnakntvy lqinslrped tavyycassr aygssrlrla dtyeywgqgt lvtvss. These two chains all show great affinity with VEGF, we used pBBR plasmid as a backbone and transfered 8IIU into Escherichia coli Nissle 1917, and finally succeeded in expressing 8IIU.

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Figure 1. 8IIU chainA features, from genebank

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Figure 2. 8IIU chainB features, from genebank

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Figure 3. 8IIU structure, from genebank

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Figure 4. Colony PCR results of pBBR-OmpA-8IIU

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Figure 5. Colony PCR results of pBBR-INP-8IIU (1)

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Figure 6. Colony PCR results of pBBR-INP-8IIU (2)

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Figure 7. Colony PCR results of pBBR-INP-8IIU (3)

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Figure 7. SDS-PAGE analysis of pBBR1MCS-OmpA-8IIU expression in Escherichia coli Nissle 1917


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
Parameters
biologyEscherichia coli Nissle 1917