Composite

Part:BBa_K243015

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)
Revision as of 02:15, 22 October 2009 by Sarah (Talk | contribs) (→‎Usage and Biology)

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Strep-DigA-Split Linker-Fok_i

This combination uses the benefits of a Streptavidin-tag for purification. It is also linked with a DigoxigeninA tag DigA. The Split Linker connects the parts and adds additional space between them to guarantee the independent function of DigA tag and the protein domain Fok_i.


Usage and Biology

This composite part is one part of our universal endonuclease and it needs another composite part like BBa_K243024 to build a functional heterodimer. The DigA tag guides the part to DNA which is hybridized with a Digoxigenin labeled oligonucleotide. The Split Linker creates a distance of 51bp between the DigA and the linked Fok_i protein domain. The Strep Tag serves as a purification tag for Streptavidin column purification.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aStrep-DigA-Split Linker-Fok_i