Composite

Part:BBa_K243013

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)
Revision as of 02:22, 22 October 2009 by Sarah (Talk | contribs) (→‎Usage and Biology)

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His-FluA-Short Linker-Fok_i

This combination uses the benefits of an His-tag for purification. It is also linked with a FluroescineA-tag. The short linker (GlySerGlyGly)connects the parts FluA and Fok_i and brigs them in spatial proximity to each other and therefor in proximity to the DNA.


Usage and Biology

This composite part is one part of our universal endonuclease and it needs another composite part like BBa_K243017 to build a functional heterodimer. The FluA tag guides the part to DNA which is hybridized with a Fluorescein labeled oligonucleotide. The short linker creates a distance of 12bp between the FluA and the linked Fok_i protein domain. The His tag serves as a purification tag for a Ni-NTA column purification.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aHis-FluA-Short Linker-Fok_i