Part:BBa_K5322033

Programmed Cell Death 1 Ligand 1 [Mus musculus]

Usage and Biology

Based on the descriptions of the mouse PD-L1 protein structure in the NCBI database (ADK70951.1, ADK70950.1, Q9NZQ7.1, AAH66841.1), as well asprogrammed cell death 1 ligand 1 precursor [Mus musculus](NP_068693.1), we optimized the DNA sequence according to the codon usage table for Escherichia coli. The optimized DNA sequence was obtained accordingly.

Protein Structure Prediction

2.1 AlphaFold 2 Prediction Results

For the PD-L1 protein, we utilized AlphaFold 2 from Google DeepMind for protein structure prediction, aiming to gain a deeper understanding of PD-L1.

AlphaFold 2 Prediction Results:

Figure 2-1 AlphaFold 2 Prediction Results

2.2 AlphaFold 3 Prediction Results

With the release of AlphaFold 3, the accuracy of protein structure prediction has been further improved. Therefore, we conducted another round of protein structure prediction.

AlphaFold 3 Prediction Results:

Figure 2-2 AlphaFold 3 Prediction Results

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 295
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 253
  Illegal PstI site found at 295
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 144
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 295
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 295
  Illegal NgoMIV site found at 684
  Illegal AgeI site found at 516
  
• 1000
  COMPATIBLE WITH RFC[1000]