Part:BBa_K5235000

BsAld

This part encodes an enzymes BsAld, from Bacillus subtilis. It is the first step in theanine synthesis, converting pyruvate to alanine. BsAld first binds with NADH to form an enzyme-cofactor complex. Then, pyruvate binds to the active site of the enzyme-cofactor complex, and NADH transfers its electrons to the pyruvate, reducing the carbonyl group (C=O) to an imine (C=NH). The imine intermediate reacts with ammonium ions to form a Schiff base intermediate, which is then hydrolyzed to produce alanine, releasing NAD⁺ and water.

[1] Cao, R., Hu, S., Lu, Y., Wang, W., Fu, Z., & Cheng, J. (2023). Fermentative Production of L-Theanine in Escherichia coli via the Construction of an Adenosine Triphosphate Regeneration System. Fermentation, 9(10), 875–875.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]