Coding

Part:BBa_K5089012:Design

Designed by: Camille Prefontaine   Group: iGEM24_UManitoba   (2024-09-29)
Revision as of 21:08, 29 September 2024 by Prefont6 (Talk | contribs)

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ABO2449


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 735
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 385
    Illegal NgoMIV site found at 559
    Illegal NgoMIV site found at 900
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence for this part is already codon-optimized for expression in E. coli.


Source

Alcanivorax borkumensis, gene number 2449

References

1. Hajighasemi, M., et al., 2016. Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases. Biomacromolecules, 17(6), 2027–2039. https://doi.org/10.1021/acs.biomac.6b00223