Device

Part:BBa_K174003:Design

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-10-04)
Revision as of 22:46, 21 October 2009 by Naw3 (Talk | contribs) (→‎Design Notes)

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Heritable, Tunable, Stochastic Switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1791
    Illegal AgeI site found at 105
    Illegal AgeI site found at 217
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pSpac and pXyl allow tunig by IPTG and Xylose. However if one wishes to control the switch with something else, the control regions can easily be replaced since they are surrounded with restriction sites.

Source

Synthesised by GeneArt

References

  1. Ham, T.S., et al., Design and Construction of a Double Inversion Recombination Switch for Heritable Sequential Genetic Memory. PLoS ONE, 2008. 3(7): p. e2815.
  2. Haynes, K., M. Broderick, et al. (2008). "Engineering bacteria to solve the Burnt Pancake Problem." Journal of Biological Engineering 2(1): 8.
  3. Kutsukake, K., et al., Two DNA Invertases Contribute to Flagellar Phase Variation in Salmonella enterica Serovar Typhimurium Strain LT2. J. Bacteriol., 2006. 188(3): p. 950-957.
  4. Veening, J.-W., H. Murray, and J. Errington, A mechanism for cell cycle regulation of sporulation initiation in Bacillus subtilis. Genes & Development, 2009. 23(16): p. 1959-1970.
  5. Kim, L., A. Mogk, et al. (1996). "A xylose-inducible Bacillus subtilis integration vector and its application." Gene 181: 71-76.
  6. Kreuzer, P., D. Gartner, et al. (1989). "Identification and sequence analysis of the Bacillus subtilis W23 xylR gene and xyl operator." J. Bacteriol. 171(7): 3840-3845.