Reporter
HREx6-yeBF

Part:BBa_K5415002:Design

Designed by: Agnese Gabriela Celma   Group: iGEM24_Latvia-Riga   (2024-09-29)
Revision as of 08:28, 30 September 2024 by Gabriela-c (Talk | contribs) (formatting)

Two sets of triple HRE containing oligonucleotide from EP0 promoter

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

Post-EcoRV CUT

gat

CYC1 promoter from pRL_yEGFP_hc

gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat

yeBFP2

ATGGTGTCAAAAGGTGAAGAATTGTTCACAGGTGTTGTTCCAATTCTAGTTGAATTAGATGGTGACGTGAATGGTCATAAGTTTTCAGTTAGAGGTGAAGGTGA

AGGTGATGCAACAAATGGTAA<p>ATTGACATTGAAGTTTATTTGTACAACCGGTAAGTTGCCAGTTCCGTGGCCAACTCTAGTTACTACATTGTCTCATGGGG

TTCAGTGCTTTGCCAGATATCCAGATCACATGAAACAACATGATT<p>TCTTCAAATCTGCTATGCCAGAAGGTTATGTTCAAGAAAGAACCATTTTTTTTAAA

GATGACGGTACATACAAAACAAGAGCTGAAGTTAAGTTCGAAGGTGATACTTTGGTTAATCGCATAGAA<p>TTGAAGGGCGTTGATTTTAAGGAAGATGGTAA

CATCCTCGGTCATAAGCTAGAATACAATTTCAATTCTCATAATATCTATATTATGGCTGTTAAACAAAAGAATGGTATTAAGGTTAATTTCAA<p>AATTAGAC

ATAATGTCGAAGATGGTTCAGTCCAATTAGCTGATCACTATCAACAAAATACCCCTATTGGTGATGGTCCTGTTTTATTGCCCGATTCTCATTATTTGTCAACT

CAATCTGTTTTAT<p>CAAAAGATCCAAACGAAAAACGTGATCATATGGTTTTGTTGGAgTTCAGAACTGCCGCAGGAATTACTTTAGGTATGGATGAACTATATAAGTAG

ADH2 terminator

gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaag

ataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttc

agcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat