Signalling

Part:BBa_I731012:Experience

Designed by: Stephanie Lo   Group: iGEM07_Harvard   (2007-10-14)
Revision as of 22:04, 21 October 2009 by Yoshimi (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I731012

User Reviews

UNIQ38856c2a40328acb-partinfo-00000000-QINU UNIQ38856c2a40328acb-partinfo-00000001-QINU Experiment : checking BBa_I731012

  1. Picked and cultured the following stab and glycerol stocks in 2mL of LB:
    1. LB-Amp, BBa_I731012, (stab, received from iGEM)
    2. LB-Amp+0.2%Glu, BBa_K084012(plac+rbs+LuxI(no LVA)), (XL10G)
    3. LB-Amp+0.2%Glu, BBa_K084007(plac+rbs+LasI(no LVA)), (XL10G)
  2. Pre-Cultured at 37°C for 12h.
  3. Added 6.25% each of the pre-cultures to new LB medium.
    1. LB-Amp, BBa_T9002, BBa_K084012(plac+rbs+LuxI(no LVA)), BBa_K084007(plac+rbs+LasI(no LVA))
  4. Cultured at 37°C for 12h
  5. Mixed the sender cells BBa_K084012 and BBa_K084007 both with BBa_I1731012 at a 1:1 ratio.
    1. BBa_I1731012 alone.
    2. BBa_K084012:BBa_I1731012=1:1
    3. BBa_K084007:BBa_BBa_I1731012=1:1
  6. Culture 12h and observe results(follow picture).
  • Funakoshi, UVGL-58 : long wave(365 nm)