Part:BBa_K5291032

pBBR1MCS2-nadM-nadK-pntA-pntB

As a plasmid, pBBR1MCS2-nadM-nadK-pntA-pntB can increase the intracellular level of NADPH by introducing four exogenous genes: nadM, nadK, pntA and pntB.

Usage and Biology

To increase the intracellular level of NADPH, we constructed the pBBR1MCS2-nadM-nadK-pntA-pntB plasmid containing four exogenous genes, including nadM, nadK, pntA, and pntB.

Fig.1 The map of plasmid pBBR1MCS2-nadM-nadK-pntA-pntB.

Since the efficiency of homologous recombination of multiple fragments was too low, we decided to ligate the two inserts first, and then ligate the inserts to the linearized vector.Here we have successfully concatenated the inserts.

Fig.2 Electropherogram of nadM-nadK-pntA-pntB PCR gel.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]