Part:BBa_K5291028

nadM: bifunctional nicotinamide-nucleotide adenylyltransferase

NadM encoded by nadM is involved in both de novo and salvage pathways of NAD biosynthesis. Bifunctional nicotinamide-nucleotide adenylyltransferase catalyzes the addition of adenylate from ATP to β-nicotinamide D-ribonucleotide, forming NAD+. We used the nadM gene to maintain intracellular NAD+ levels.

Usage and Biology

In carbon fixation module we constructed pBBR1MCS2-nadM-nadK-pntA-pntB to improve the intracellular NADPH and NADP⁺ level.

Fig.1 The map of plasmid pBBR1MCS2-nadM-nadK-pntA-pntB.

We successfully cloned the nadM-nadK gene by PCR.

Fig.2 Electropherogram of nadM-nadK PCR gel.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]