Coding

Part:BBa_K5291027

Designed by: Haosheng Lin   Group: iGEM24_BNUZH-China   (2024-09-25)
Revision as of 00:55, 2 October 2024 by Kendy (Talk | contribs)

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nadK: nicotinamide adenine dinucleotide kinase

nadK encodes NAD kinases(NADK). NADK can use ATP as their phosphoryl donor to phosphorylate NAD+. We used it to increase the intracellular levels of NADP+.

Usage and Biology

In carbon fixation module we constructed pBBR1MCS2-nadM-nadK-pntA-pntB to improve the intracellular NADPH and NADP+ level.

Fig.1 The map of plasmid pBBR1MCS2-nadM-nadK-pntA-pntB.

We successfully cloned the nadM-nadK gene by PCR.

Fig.2 Electropherogram of nadM-nadK PCR gel.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None