Part:BBa_K180010

Rock-paper-scissors - Rock primary plasmid

Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand.

BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180011) has no effect over mCherry or LasI expression in BBa_K180012.

mCherry and LasI are generated under regulation by tac pR, whilst RhlR is produced constitutively.

Forms the 'rock' primary plasmid in Team:Southampton's rock-paper-scissors project design.

Usage and Biology

This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).

To function as the 'Rock' in the rock-paper-scissors design by Team:Southampton, the part has to be used in conjunction with K180013, and pairs K180014 & K180016, K180017 & K180020.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 2760
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 893
  Illegal NheI site found at 916
  Illegal PstI site found at 2760
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 622
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 2760
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 2760
  Illegal AgeI site found at 3365
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 147