Device

Part:BBa_K174011:Design

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-10-13)
Revision as of 21:17, 21 October 2009 by Naw3 (Talk | contribs) (→‎References)

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IPTG inducable kinA Bacillus sporulation trigger


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In B. subtilis, out of the five kinases KinA-E only KinA, KinB and KinC kinases trigger sporulation. KinD and KinE do not phosphorylate Spo0F, hence do not effect sporulation. We therefore chose to overexpress the kinA gene for our device.

This part has received basic characterisation.

pSpac was included for testing purposes. However we designed our promoter with flanking sites to allow different inducible promoters to be used.

Source

Synthesised.

References

  1. Veening, J.-W., W. K. Smits, et al. (2008). "Bistability, Epigenetics, and Bet-Hedging in Bacteria." Annual Review of Microbiology 62(1): 193-210.
  2. Fujita, M. and R. Losick (2005). "Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A." Genes & Development 19(18): 2236-2244.