Composite
LongLinker

Part:BBa_K243009:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)
Revision as of 00:50, 22 October 2009 by Sarah (Talk | contribs) (→‎Design Notes)

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Long Linker-Fok_i


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The length of this linker is useful to produce a connection to another part and produce great distance between two parts. The long distance produced by the linker reduces possible sterical interferences and increases the distance from the protein Fok_i to the DNA when it is linked to a DNA binding protein. We had to prove the choice of distance between two parts and the distance between our protein domain Fok_a and Fok_i.
Designed according to the RFC 25.

Commented GenBank file

Source

Combinated by cloning.

References